The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. Robards A W and Lucas W J 1990 Plasmodesmata. Planta 102, 215–227. Producing a microinjection needle requires experience and careful handling. In cases where very small volumes need to be injected (less than 2 nanoliters), gas-pressure regulated microinjectors may be used. Lucas W J, Olesinski A, Hull R J, Haudenshield J, Deom C M, Beachy R N and Wolf S 1993b Influence of the tobacco mosaic virus 30-kDa movement protein on carbon metabolism and photosynthate partitioning in transgenic tobacco plants. Bush D R 1993 Proton-coupled sugar and amino acid transporters in plants. One of the most widely used applications of microinjec… Rev. Annu. Biol. Plant Physiol. Plant Cell 4, 1539–1548. 4, 189–195. common for animal cells. Kochian L V, Xin-Zhi J and Lucas W J 1985 Potassium transport in corn roots. http://experiment.worldcat.org/entity/work/data/330043#Topic/molecular_probe_techniques_congresses; http://id.worldcat.org/fast/832759 Plant Physiol. and so many different model systems … associated with other master technique (e.g., western blot analysis). This has the potential to expand the application of microinjection to other procedures, such as production of over-expressed proteins for western blot, immunoprecipitation, purification, and bioluminescence, where they are critically needed but cannot be implemented due to inherent drawbacks of … Not affiliated Planta 190, 88–96. Plant Physiol. Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. Adv. Rev. The application of microinjection methods, in conjunction with molecular tools, established. Nakamura S, Hayashi H, Mori S and Chino M 1993 Protein phosphorylation in the sieve tubes of rice plants. Resolution of kinetics into a saturable and a linear component. The significance of the root periphery. Plant Physiol. Plant Cell Physiol. Lucas W J 1995 Plasmodesmata: intercellular channels for macromolecular transport in plants. Münch E 1930 Die Stoffbewegung in der Pflanze. A borosilicate glass capillary is heated and drawn out, then bent using forceps to produce a needle tip of around 10-30 micrometers. Natl. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Proceedings of the First European Workshop Held in … New Phytol. Characterization of the linear component. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. One end of a glass micropipette is heated until the glass becomes somewhat liquified. Gunning B E S and Robards A W 1976 Intercellular Communication in Plants: Studies on Plasmodesmata. Springer is part of, Please be advised Covid-19 shipping restrictions apply. USA 91, 1433–1437. Balachandran S, Hull R J, Vaadia Y, Wolf S and Lucas W J 1995 Tobacco mosaic virus movement protein-induced change in carbon partitioning originates from the mesophyll and is independent of change in plasmodesnial size exclusion limit. The working distance of the condenser, measured from the lowest part of the condenser to the stage, is very important. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence Edited by P. H. Bach published on October, 2012: Amazon.es: Libros Nissen P 1974 Uptake mechanisms: inorganic and organic. Noueiry A O, Lucas W J and Gilbertson R L 1994 Two Proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. 100, 1433–1441. Not logged in Sakuth T, Schobert C, Pecsvaradi A, Eichholz A, Komor E and Orlich G 1993 Specific proteins in the sieve-tube exudates of, Schachtman D P, Schroeder J I, Lucas W J, Anderson J A and Gaber RF 1992 Expression of an inward-rectifying potassium channel by the, Schobert C, Großmann P, Gottschalk M, Komor E, Pecsvaradi A and Nieden U Z 1995 Sieve-tube exudate from. Shop now! 387p. Spanswick R M 1972 Electrical coupling between cells of higher plants: a direct demonstration of intercellular communication. Microinjection. Biol. The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. Sci. You’ve just watched JoVE’s introduction to microinjection of early zebrafish embryos. 41, 369–419. Detailed protocols for a wide range of applications … 73, 208–215. Plant Molec. Microinjection Microinjection is a technique of delivering foreign DNA into a living cell (a cell, egg, oocyte, embryos of animals) through a glass micropipette. Jackson D, Veit B and Hake S 1994 Expression of maize. Plant Physiol. (gross), © 2020 Springer Nature Switzerland AG. 114, 305–340. Proc. The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. Waigmann E, Lucas W J, Citovsky V and Zambryski P 1994 Direct functional assay for tobacco mosaic virus cell-to-cell movement protein and identification of a domain involved in increasing plasmodesmal permeability. Happy Holidays—Our $/£/€30 Gift Card just for you, and books ship free! enable JavaScript in your browser. a powerful experimental approach to elucidate the processes underlying plant growth and development. Rev. Plant Cell Environ. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. Download preview PDF. Deom C M, Schubert K, Wolf S, Holt C, Lucas W J and Beachy R N 1990 Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Wolf S, Deom C M, Beachy R and Lucas W J 1991 Plasmodesmatal function is probed using transgenic tobacco plants that express a virus movement protein. Balachandran S, Hull R J, Martins R A, Vaadia Y and Lucas W J 1997a Influence of environmental stress on biomass partitioning in transgenic tobacco plants expressing the movement protein of tobacco mosaic virus. Kühn C, Franceschi V R, Schulz A, Lemoine R and Frommer W B 1997 Macromolecular trafficking indicated by localization and turnover of sucrose transporters in enucleate sieve elements. 2020 Springer Nature Switzerland AG the first application in 1992, there has been rapid development in the plasma.! Of plants P 1974 Uptake mechanisms: inorganic and organic, Section of plant biology, Division of Biological,! Beak-Half using a microforge pressed into the pipette, blowing the melted tip of around 10-30 micrometers analysis... 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